Journal: Animals : an Open Access Journal from MDPI

Article Title: FABP3 Mediates Lipid Droplet Accumulation and Adhesive Capacity in Bovine Endometrial Epithelial Cells via PGE 2 /PTGER4/PPAR Axis

doi: 10.3390/ani15233417

Figure Lengend Snippet: PGE 2 binds to PTGER4 to regulate FABP3 expression in bEECs: ( A ) The difference in PTGER ( PTGER1 , PTGER2 , PTGER3 and PTGER4 ) expression in bEECs after treatment with P 4 and/or PGE 2 . ( B – D ) The representative images and analysis of PTGER2 and PTGER4 expression in bEECs after treatment with P 4 and/or PGE 2 . ( E , F ) The representative images and analysis of PTGER2 expression in bEECs between shN and shPTGER2 groups after treatment with DMSO or P 4 + PGE 2 . ( G , H ) The representative images and analysis of PTGER4 expression in bEECs between shN and shPTGER4 groups after treatment with DMSO or P 4 + PGE 2 . ( I ) The analysis of FABP3 expression in bEECs after treatment with DMSO, PGE 2 or the inhibitor of PTGER4 ( GW627368 ). The data are means ± SEM of three independent experiments. Statistically significant differences are indicated by asterisks: ** p < 0.01, ns p > 0.05. Bars with different letters are significantly different ( p < 0.05).

Article Snippet: Then, PGE 2 (10 −8 –10 −6 M; 14010, Cayman, Ann Arbor, MI, USA) was added to the medium, and the cells were cultured for 24 h. For inhibitor treatment, 5 and 10 μM PTGER2 inhibitor (TG-155; MedChemExpress, Newark, NJ, USA) or 5 and 10 μM PTGER4 inhibitor ( GW627368 ; Selleck, Houston, TX, USA) were added to the medium 1 h prior to the PGE 2 treatment.

Techniques: Expressing